Background: Many studies have investigated the effect of spiritual intelligence on various psychological variables, but research on the impact of spiritual intelligence on spiritual intelligence, psychological well-being, anxiety, and depression remains largely ignored. Aims: The purpose of this study was to investigate the effect of education based on Bowl spiritual intelligence promotion on spiritual intelligence, psychological well-being, anxiety and depression in students. Method: It was a quasi-experimental type. The statistical population of this study was Isfahan University students, 40 of whom were selected as available sample in 1977 and were randomly assigned to education or control group. Research tools include the Reef Psychological Well-being Questionnaire (2002), Anxiety (1993) and Beck Depression Inventory (1996), and King's Spiritual Intelligence (2008), and Bowl's Spiritual Intelligence Training (2004). Data were analyzed using multivariate analysis of covariance. Results: Bowl spiritual intelligence training in the educational group improved spiritual intelligence (P<0/001), reduced anxiety (P<0/05), decreased depression (P<0/001) but had a significant effect on psychological well-being (P<0/001). Did not have (P<0/001). Conclusions: Spiritual intelligence training improved spiritual intelligence and reduced anxiety and depression in students.