Background: research shows organizational bullying can create emotional and psychological damages in employees. Could emotion regulation training help to decrease damages? Aims: The present study was conducted aiming to investigate the effect of Emotion Regulation training on perceived bullying and job performance of female employees at the organization of physical education. Method: It was a quasi-experimental study with pretest, posttest and control group design. The statistical population included all women working at the organization of physical education in the province of Isfahan who were working in the autumn of 2018. 30 female employees at the organization of physical education in the province of Isfahan were selected through non-random purposive sampling method and randomly replaced into experimental and control groups. Experimental group received 8 session of group emotional regulation training (Gross & Thompson, 2007). The applied questionnaires included perceived organizational bullying (researcher-made) questionnaire and job performance questionnaire (Hersi and Goldsmith, 1990). The data from the study were analyzed through ANCOVA method. Results: The result showed that emotion regulation training has significantly influenced perceived bullying and job performance of female employees at the organization of physical education (p<0/0001). The statistical effect size of emotion regulation training on perceived bullying and job performance of female employees at the organization of physical education was 67% and 53% respectively. Conclusions: based on the findings of the study it can be concluded that teaching emotion regulation techniques can increase job performance and decrease perceived bullying among female employees in organization of physical education.